SNAP output for PDB entry 3p49 [SNAP web server]

PDB-id

3p49; SNAP-derived features in text and JSON formats; DNAproDB

Class

RNA binding protein-RNA

Method

X-ray (3.55 Å)

Summary

Crystal structure of a glycine riboswitch from fusobacterium nucleatum

Reference

Butler EB, Xiong Y, Wang J, Strobel SA (2011): "Structural basis of cooperative ligand binding by the glycine riboswitch." Chem.Biol., 18, 293-298. doi: 10.1016/j.chembiol.2011.01.013.

Abstract

The glycine riboswitch regulates gene expression through the cooperative recognition of its amino acid ligand by a tandem pair of aptamers. A 3.6 Å crystal structure of the tandem riboswitch from the glycine permease operon of Fusobacterium nucleatum reveals the glycine binding sites and an extensive network of interactions, largely mediated by asymmetric A-minor contacts, that serve to communicate ligand binding status between the aptamers. These interactions provide a structural basis for how the glycine riboswitch cooperatively regulates gene expression.