SNAP output for PDB entry 3s8q [SNAP web server]

PDB-id

3s8q; SNAP-derived features in text and JSON formats; DNAproDB

Class

protein binding-DNA

Method

X-ray (2.1 Å)

Summary

Crystal structure of the r-m controller protein c.esp1396i ol operator complex

Reference

McGeehan JE, Ball NJ, Streeter SD, Thresh SJ, Kneale GG (2012): "Recognition of dual symmetry by the controller protein C.Esp1396I based on the structure of the transcriptional activation complex." Nucleic Acids Res., 40, 4158-4167. doi: 10.1093/nar/gkr1250.

Abstract

The controller protein C.Esp1396I regulates the timing of gene expression of the restriction-modification (RM) genes of the RM system Esp1396I. The molecular recognition of promoter sequences by such transcriptional regulators is poorly understood, in part because the DNA sequence motifs do not conform to a well-defined symmetry. We report here the crystal structure of the controller protein bound to a DNA operator site. The structure reveals how two different symmetries within the operator are simultaneously recognized by the homo-dimeric protein, underpinned by a conformational change in one of the protein subunits. The recognition of two different DNA symmetries through movement of a flexible loop in one of the protein subunits may represent a general mechanism for the recognition of pseudo-symmetric DNA sequences.