Summary information and primary citation

PDB-id
3uo7; SNAP-derived features in text and JSON formats; DNAproDB
Class
hydrolase-DNA
Method
X-ray (3.002 Å)
Summary
Crystal structure of human thymine DNA glycosylase bound to substrate 5-carboxylcytosine
Reference
Zhang L, Lu X, Lu J, Liang H, Dai Q, Xu GL, Luo C, Jiang H, He C (2012): "Thymine DNA glycosylase specifically recognizes 5-carboxylcytosine-modified DNA." Nat.Chem.Biol., 8, 328-330. doi: 10.1038/nchembio.914.
Abstract
Human thymine DNA glycosylase (hTDG) efficiently excises 5-carboxylcytosine (5caC), a key oxidation product of 5-methylcytosine in genomic DNA, in a recently discovered cytosine demethylation pathway. We present here the crystal structures of the hTDG catalytic domain in complex with duplex DNA containing either 5caC or a fluorinated analog. These structures, together with biochemical and computational analyses, reveal that 5caC is specifically recognized in the active site of hTDG, supporting the role of TDG in mammalian 5-methylcytosine demethylation.

Cartoon-block schematics in six views (download the tarball)

PyMOL session file Download PDB file View in 3Dmol.js