Summary information and primary citation
- PDB-id
-
3wkj;
DSSR-derived features in text and
JSON formats; DNAproDB
- Class
- structural protein-DNA
- Method
- X-ray (2.8 Å)
- Summary
- The nucleosome containing human tsh2b
- Reference
-
Urahama T, Horikoshi N, Osakabe A, Tachiwana H,
Kurumizaka H (2014): "Structure
of human nucleosome containing the testis-specific
histone variant TSH2B." Acta
Crystallogr.,Sect.F, 70, 444-449.
doi: 10.1107/S2053230X14004695.
- Abstract
- The human histone H2B variant TSH2B is highly expressed
in testis and may function in the chromatin transition
during spermatogenesis. In the present study, the crystal
structure of the human testis-specific nucleosome
containing TSH2B was determined at 2.8 Å resolution. A
local structural difference between TSH2B and canonical H2B
in nucleosomes was detected around the TSH2B-specific
amino-acid residue Ser85. The TSH2B Ser85 residue does not
interact with H4 in the nucleosome, but in the canonical
nucleosome the H2B Asn84 residue (corresponding to the
TSH2B Ser85 residue) forms water-mediated hydrogen bonds
with the H4 Arg78 residue. In contrast, the other
TSH2B-specific amino-acid residues did not induce any
significant local structural changes in the TSH2B
nucleosome. These findings may provide important
information for understanding how testis-specific histone
variants form nucleosomes during spermatogenesis.
