Summary information and primary citation
- PDB-id
- 4bwj; SNAP-derived features in text and JSON formats;
DNAproDB
- Class
- transferase-DNA
- Method
- X-ray (1.55 Å)
- Summary
- Klentaq mutant in complex with DNA and ddctp
- Reference
- Blatter N, Bergen K, Nolte O, Welte W, Diederichs K, Mayer J, Wieland M, Marx A (2013): "Structure and Function of an RNA-Reading Thermostable DNA Polymerase." Angew.Chem.Int.Ed.Engl., 52, 11935. doi: 10.1002/ANIE.201306655.
- Abstract
- Unfaithful: The structure of a DNA-dependent DNA polymerase, whose parental ancestor has no significant reverse transcriptase activity, was obtained for the first time incorporating a nucleotide against an RNA template and then structurally characterized. The DNA polymerase variant has significant reverse transcriptase activity that allows its application in PCR-based molecular diagnostics.
