SNAP output for PDB entry 4ce4 [SNAP web server]

PDB-id

4ce4; SNAP-derived features in text and JSON formats; DNAproDB

Class

ribosome

Method

cryo-EM (4.9 Å)

Summary

39s large subunit of the porcine mitochondrial ribosome

Reference

Greber BJ, Boehringer D, Leitner A, Bieri P, Voigts-Hoffmann F, Erzberger JP, Leibundgut M, Aebersold R, Ban N (2014): "Architecture of the Large Subunit of the Mammalian Mitochondrial Ribosome." Nature, 505, 515. doi: 10.1038/NATURE12890.

Abstract

Mitochondrial ribosomes synthesize a number of highly hydrophobic proteins encoded on the genome of mitochondria, the organelles in eukaryotic cells that are responsible for energy conversion by oxidative phosphorylation. The ribosomes in mammalian mitochondria have undergone massive structural changes throughout their evolution, including ribosomal RNA shortening and acquisition of mitochondria-specific ribosomal proteins. Here we present the three-dimensional structure of the 39S large subunit of the porcine mitochondrial ribosome determined by cryo-electron microscopy at 4.9 Å resolution. The structure, combined with data from chemical crosslinking and mass spectrometry experiments, reveals the unique features of the 39S subunit at near-atomic resolution and provides detailed insight into the architecture of the polypeptide exit site. This region of the mitochondrial ribosome has been considerably remodelled compared to its bacterial counterpart, providing a specialized platform for the synthesis and membrane insertion of the highly hydrophobic protein components of the respiratory chain.