Summary information and primary citation
- PDB-id
- 4g7h; SNAP-derived features in text and JSON formats;
DNAproDB
- Class
- transcription, transferase-DNA
- Method
- X-ray (2.9 Å)
- Summary
- Crystal structure of thermus thermophilus transcription initiation complex
- Reference
- Zhang Y, Feng Y, Chatterjee S, Tuske S, Ho MX, Arnold E, Ebright RH (2012): "Structural basis of transcription initiation." Science, 338, 1076-1080. doi: 10.1126/science.1227786.
- Abstract
- During transcription initiation, RNA polymerase (RNAP) binds and unwinds promoter DNA to form an RNAP-promoter open complex. We have determined crystal structures at 2.9 and 3.0 Å resolution of functional transcription initiation complexes comprising Thermus thermophilus RNA polymerase, σ(A), and a promoter DNA fragment corresponding to the transcription bubble and downstream dsDNA of the RNAP-promoter open complex. The structures show that σ recognizes the -10 element and discriminator element through interactions that include the unstacking and insertion into pockets of three DNA bases, and that RNAP recognizes the -4/+2 region through interactions that include the unstacking and insertion into a pocket of the +2 base. The structures further show that interactions between σ and template-strand ssDNA preorganize template-strand ssDNA to engage the RNAP active center.
