Summary information and primary citation
- PDB-id
- 4gle; SNAP-derived features in text and JSON formats;
DNAproDB
- Class
- hydrolase-DNA
- Method
- X-ray (2.7 Å)
- Summary
- Sacuvde in complex with 6-4pp-containing DNA
- Reference
- Meulenbroek EM, Peron Cane C, Jala I, Iwai S, Moolenaar GF, Goosen N, Pannu NS (2013): "UV damage endonuclease employs a novel dual-dinucleotide flipping mechanism to recognize different DNA lesions." Nucleic Acids Res., 41, 1363-1371. doi: 10.1093/nar/gks1127.
- Abstract
- Repairing damaged DNA is essential for an organism's survival. UV damage endonuclease (UVDE) is a DNA-repair enzyme that can recognize and incise different types of damaged DNA. We present the structure of Sulfolobus acidocaldarius UVDE on its own and in a pre-catalytic complex with UV-damaged DNA containing a 6-4 photoproduct showing a novel 'dual dinucleotide flip' mechanism for recognition of damaged dipyrimidines: the two purines opposite to the damaged pyrimidine bases are flipped into a dipurine-specific pocket, while the damaged bases are also flipped into another cleft.
