Summary information and primary citation

PDB-id
4ii9; SNAP-derived features in text and JSON formats; DNAproDB
Class
transferase-peptide-RNA
Method
X-ray (1.66 Å)
Summary
Crystal structure of weissella viridescens femxvv non-ribosomal amino acid transferase in complex with a peptidyl-RNA conjugate
Reference
Fonvielle M, Li de La Sierra-Gallay I, El-Sagheer AH, Lecerf M, Patin D, Mellal D, Mayer C, Blanot D, Gale N, Brown T, van Tilbeurgh H, Etheve-Quelquejeu M, Arthur M (2013): "The Structure of FemXWv in Complex with a Peptidyl-RNA Conjugate: Mechanism of Aminoacyl Transfer from Ala-tRNA(Ala) to Peptidoglycan Precursors." Angew.Chem.Int.Ed.Engl., 52, 7278-7281. doi: 10.1002/anie.201301411.
Abstract
To gain insight into the catalytic mechanism of non-ribosomal amino acid transferases, peptidyl-RNA conjugates were synthesized for co-crystallization with FemXWv of Weissella viridescens, which transfers L-Ala from Ala-tRNA(Ala) to the peptidoglycan precursor UDP-MurNAc-pentapeptide. The structure of the resulting complex and mutational studies revealed the mechanism by which FemXWv binds its substrates for substrate-assisted catalysis and stabilization of the tetrahedral intermediate.

Cartoon-block schematics in six views (download the tarball)

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