Summary information and primary citation
- PDB-id
- 4lg2; SNAP-derived features in text and JSON formats;
DNAproDB
- Class
- RNA binding protein-RNA
- Method
- X-ray (2.7 Å)
- Summary
- Crystal structure of reston ebola virus vp35 RNA binding domain bound to 12-bp dsrna
- Reference
- Bale S, Julien JP, Bornholdt ZA, Krois AS, Wilson IA, Saphire EO (2013): "Ebolavirus VP35 coats the backbone of double-stranded RNA for interferon antagonism." J.Virol., 87, 10385-10388. doi: 10.1128/JVI.01452-13.
- Abstract
- Recognition of viral double-stranded RNA (dsRNA) activates interferon production and immune signaling in host cells. Crystal structures of ebolavirus VP35 show that it caps dsRNA ends to prevent sensing by pattern recognition receptors such as RIG-I. In contrast, structures of marburgvirus VP35 show that it primarily coats the dsRNA backbone. Here, we demonstrate that ebolavirus VP35 also coats the dsRNA backbone in solution, although binding to the dsRNA ends probably constitutes the initial binding event.
