Summary information and primary citation

PDB-id
4m30; SNAP-derived features in text and JSON formats; DNAproDB
Class
hydrolase-RNA
Method
X-ray (2.501 Å)
Summary
Crystal structure of rnase iii complexed with double-stranded RNA and amp (type ii cleavage)
Reference
Court DL, Gan J, Liang YH, Shaw GX, Tropea JE, Costantino N, Waugh DS, Ji X (2013): "RNase III: Genetics and Function; Structure and Mechanism." Annu. Rev. Genet., 47, 405-431. doi: 10.1146/annurev-genet-110711-155618.
Abstract
RNase III is a global regulator of gene expression in Escherichia coli that is instrumental in the maturation of ribosomal and other structural RNAs. We examine here how RNase III itself is regulated in response to growth and other environmental changes encountered by the cell and how, by binding or processing double-stranded RNA (dsRNA) intermediates, RNase III controls the expression of genes. Recent insight into the mechanism of dsRNA binding and processing, gained from structural studies of RNase III, is reviewed. Structural studies also reveal new cleavage sites in the enzyme that can generate longer 3' overhangs.

Cartoon-block schematics in six views (download the tarball)

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