SNAP output for PDB entry 4oau [SNAP web server]

PDB-id

4oau; SNAP-derived features in text and JSON formats; DNAproDB

Class

hydrolase-RNA

Method

X-ray (2.6 Å)

Summary

Complete human rnase l in complex with biological activators.

Reference

Han Y, Donovan J, Rath S, Whitney G, Chitrakar A, Korennykh A (2014): "Structure of human RNase L reveals the basis for regulated RNA decay in the IFN response." Science, 343, 1244-1248. doi: 10.1126/science.1249845.

Abstract

One of the hallmark mechanisms activated by type I interferons (IFNs) in human tissues involves cleavage of intracellular RNA by the kinase homology endoribonuclease RNase L. We report 2.8 and 2.1 angstrom crystal structures of human RNase L in complexes with synthetic and natural ligands and a fragment of an RNA substrate. RNase L forms a crossed homodimer stabilized by ankyrin (ANK) and kinase homology (KH) domains, which positions two kinase extension nuclease (KEN) domains for asymmetric RNA recognition. One KEN protomer recognizes an identity nucleotide (U), whereas the other protomer cleaves RNA between nucleotides +1 and +2. The coordinated action of the ANK, KH, and KEN domains thereby provides regulated, sequence-specific cleavage of viral and host RNA targets by RNase L.