SNAP output for PDB entry 4uer [SNAP web server]

PDB-id

4uer; SNAP-derived features in text and JSON formats; DNAproDB

Class

translation

Method

cryo-EM (6.47 Å)

Summary

40s-eif1-eif1a-eif3-eif3j translation initiation complex from lachancea kluyveri

Reference

Aylett CHS, Boehringer D, Erzberger JP, Schaefer T, Ban N (2015): "Structure of a Yeast 40S-Eif1-Eif1A-Eif3-Eif3J Initiation Complex." Nat.Struct.Mol.Biol., 22, 269. doi: 10.1038/NSMB.2963.

Abstract

Eukaryotic translation initiation requires cooperative assembly of a large protein complex at the 40S ribosomal subunit. We have resolved a budding yeast initiation complex by cryo-EM, allowing placement of prior structures of eIF1, eIF1A, eIF3a, eIF3b and eIF3c. Our structure highlights differences in initiation-complex binding to the ribosome compared to that of mammalian eIF3, demonstrates a direct contact between eIF3j and eIF1A and reveals the network of interactions between eIF3 subunits.