SNAP output for PDB entry 5e5a [SNAP web server]

PDB-id

5e5a; SNAP-derived features in text and JSON formats; DNAproDB

Class

protein binding-viral protein-DNA

Method

X-ray (2.809 Å)

Summary

Crystal structure of the chromatin-tethering domain of human cytomegalovirus ie1 protein bound to the nucleosome core particle

Reference

Fang Q, Chen P, Wang M, Fang J, Yang N, Li G, Xu RM (2016): "Human cytomegalovirus IE1 protein alters the higher-order chromatin structure by targeting the acidic patch of the nucleosome." Elife, 5. doi: 10.7554/eLife.11911.

Abstract

Human cytomegalovirus (hCMV) immediate early 1 (IE1) protein associates with condensed chromatin of the host cell during mitosis. We have determined the structure of the chromatin-tethering domain (CTD) of IE1 bound to the nucleosome core particle, and discovered that IE1-CTD specifically interacts with the H2A-H2B acidic patch and impairs the compaction of higher-order chromatin structure. Our results suggest that IE1 loosens up the folding of host chromatin during hCMV infections.