SNAP output for PDB entry 5f7q [SNAP web server]

PDB-id

5f7q; SNAP-derived features in text and JSON formats; DNAproDB

Class

transcription-DNA

Method

X-ray (2.4 Å)

Summary

Rok repressor lmo0178 from listeria monocytogenes bound to operator

Reference

Light SH, Cahoon LA, Halavaty AS, Freitag NE, Anderson WF (2016): "Structure to function of an alpha-glucan metabolic pathway that promotes Listeria monocytogenes pathogenesis." Nat Microbiol, 2, 16202. doi: 10.1038/nmicrobiol.2016.202.

Abstract

Here we employ a 'systems structural biology' approach to functionally characterize an unconventional α-glucan metabolic pathway from the food-borne pathogen Listeria monocytogenes (Lm). Crystal structure determination coupled with basic biochemical and biophysical assays allowed for the identification of anabolic, transport, catabolic and regulatory portions of the cycloalternan pathway. These findings provide numerous insights into cycloalternan pathway function and reveal the mechanism of repressor, open reading frame, kinase (ROK) transcription regulators. Moreover, by developing a structural overview we were able to anticipate the cycloalternan pathway's role in the metabolism of partially hydrolysed starch derivatives and demonstrate its involvement in Lm pathogenesis. These findings suggest that the cycloalternan pathway plays a role in interspecies resource competition-potentially within the host gastrointestinal tract-and establish the methodological framework for characterizing bacterial systems of unknown function.