SNAP output for PDB entry 5fur [SNAP web server]

PDB-id

5fur; SNAP-derived features in text and JSON formats; DNAproDB

Class

transcription

Method

cryo-EM (8.5 Å)

Summary

Structure of human tfiid-iia bound to core promoter DNA

Reference

Louder RK, He Y, Lopez-Blanco JR, Fang J, Chacon P, Nogales E (2016): "Structure of Promoter-Bound TFIID and Model of Human Pre-Initiation Complex Assembly." Nature, 531, 604. doi: 10.1038/NATURE17394.

Abstract

The general transcription factor IID (TFIID) plays a central role in the initiation of RNA polymerase II (Pol II)-dependent transcription by nucleating pre-initiation complex (PIC) assembly at the core promoter. TFIID comprises the TATA-binding protein (TBP) and 13 TBP-associated factors (TAF1-13), which specifically interact with a variety of core promoter DNA sequences. Here we present the structure of human TFIID in complex with TFIIA and core promoter DNA, determined by single-particle cryo-electron microscopy at sub-nanometre resolution. All core promoter elements are contacted by subunits of TFIID, with TAF1 and TAF2 mediating major interactions with the downstream promoter. TFIIA bridges the TBP-TATA complex with lobe B of TFIID. We also present the cryo-electron microscopy reconstruction of a fully assembled human TAF-less PIC. Superposition of common elements between the two structures provides novel insights into the general role of TFIID in promoter recognition, PIC assembly, and transcription initiation.