SNAP output for PDB entry 5gxi [SNAP web server]

PDB-id

5gxi; SNAP-derived features in text and JSON formats; DNAproDB

Class

RNA binding protein-RNA

Method

X-ray (1.85 Å)

Summary

Structure of the gemin5 wd40 domain in complex with aauuuuugag

Reference

Xu C, Ishikawa H, Izumikawa K, Li L, He H, Nobe Y, Yamauchi Y, Shahjee HM, Wu X-H, Yu Y-T, Isobe T, Takahashi N, Min J (2016): "Structural insights into Gemin5-guided selection of pre-snRNAs for snRNP assembly." Genes Dev., 30, 2376-2390. doi: 10.1101/gad.288340.116.

Abstract

In cytoplasm, the survival of motor neuron (SMN) complex delivers pre-small nuclear RNAs (pre-snRNAs) to the heptameric Sm ring for the assembly of the ring complex on pre-snRNAs at the conserved Sm site [A(U)4-6G]. Gemin5, a WD40 protein component of the SMN complex, is responsible for recognizing pre-snRNAs. In addition, Gemin5 has been reported to specifically bind to the m(7)G cap. In this study, we show that the WD40 domain of Gemin5 is both necessary and sufficient for binding the Sm site of pre-snRNAs by isothermal titration calorimetry (ITC) and mutagenesis assays. We further determined the crystal structures of the WD40 domain of Gemin5 in complex with the Sm site or m(7)G cap of pre-snRNA, which reveal that the WD40 domain of Gemin5 recognizes the Sm site and m(7)G cap of pre-snRNAs via two distinct binding sites by respective base-specific interactions. In addition, we also uncovered a novel role of Gemin5 in escorting the truncated forms of U1 pre-snRNAs for proper disposal. Overall, the elucidated Gemin5 structures will contribute to a better understanding of Gemin5 in small nuclear ribonucleic protein (snRNP) biogenesis as well as, potentially, other cellular activities.