Summary information and primary citation
- PDB-id
- 5msf; SNAP-derived features in text and JSON formats;
DNAproDB
- Class
- virus-RNA
- Method
- X-ray (2.8 Å)
- Summary
- Ms2 protein capsid-RNA complex
- Reference
- Rowsell S, Stonehouse NJ, Convery MA, Adams CJ, Ellington AD, Hirao I, Peabody DS, Stockley PG, Phillips SE (1998): "Crystal structures of a series of RNA aptamers complexed to the same protein target." Nat.Struct.Biol., 5, 970-975. doi: 10.1038/2946.
- Abstract
- We have determined the crystal structures, at 2.8 A resolution, of two different RNA aptamers, each bound to MS2 coat protein. One of the aptamers contains a non-Watson-Crick base pair, while the other is missing one of the unpaired adenines that make sequence-specific contacts in the wild-type complex. Despite these differences, the RNA aptamers bind in the same location on the protein as the wild-type translational operator. Comparison of these new structures with other MS2-RNA complexes allows us to refine further the definition of the minimal recognition elements and suggests a possible application of the MS2 system for routine structure determination of small nucleic acid motifs.
