Summary information and primary citation
- PDB-id
- 5n61; SNAP-derived features in text and JSON formats;
DNAproDB
- Class
- transferase
- Method
- cryo-EM (3.4 Å)
- Summary
- RNA polymerase i initially transcribing complex
- Reference
- Engel C, Gubbey T, Neyer S, Sainsbury S, Oberthuer C, Baejen C, Bernecky C, Cramer P (2017): "Structural Basis of RNA Polymerase I Transcription Initiation." Cell, 169, 120-131.e22. doi: 10.1016/j.cell.2017.03.003.
- Abstract
- Transcription initiation at the ribosomal RNA promoter requires RNA polymerase (Pol) I and the initiation factors Rrn3 and core factor (CF). Here, we combine X-ray crystallography and cryo-electron microscopy (cryo-EM) to obtain a molecular model for basal Pol I initiation. The three-subunit CF binds upstream promoter DNA, docks to the Pol I-Rrn3 complex, and loads DNA into the expanded active center cleft of the polymerase. DNA unwinding between the Pol I protrusion and clamp domains enables cleft contraction, resulting in an active Pol I conformation and RNA synthesis. Comparison with the Pol II system suggests that promoter specificity relies on a distinct "bendability" and "meltability" of the promoter sequence that enables contacts between initiation factors, DNA, and polymerase.
