Summary information and primary citation
- PDB-id
- 5sva; SNAP-derived features in text and JSON formats;
DNAproDB
- Class
- transcription, transferase-DNA
- Method
- cryo-EM (15.3 Å)
- Summary
- Mediator-RNA polymerase ii pre-initiation complex
- Reference
- Robinson PJ, Trnka MJ, Bushnell DA, Davis RE, Mattei PJ, Burlingame AL, Kornberg RD (2016): "Structure of a Complete Mediator-RNA Polymerase II Pre-Initiation Complex." Cell, 166, 1411-1422.e16. doi: 10.1016/j.cell.2016.08.050.
- Abstract
- A complete, 52-protein, 2.5 million dalton, Mediator-RNA polymerase II pre-initiation complex (Med-PIC) was assembled and analyzed by cryo-electron microscopy and by chemical cross-linking and mass spectrometry. The resulting complete Med-PIC structure reveals two components of functional significance, absent from previous structures, a protein kinase complex and the Mediator-activator interaction region. It thereby shows how the kinase and its target, the C-terminal domain of the polymerase, control Med-PIC interaction and transcription.
