SNAP output for PDB entry 5zd4 [SNAP web server]

PDB-id

5zd4; SNAP-derived features in text and JSON formats; DNAproDB

Class

transcription

Method

X-ray (2.17 Å)

Summary

Crystal structure of mbp-fused bil1-bzr1 in complex with double-stranded DNA

Reference

Nosaki S, Miyakawa T, Xu Y, Nakamura A, Hirabayashi K, Asami T, Nakano T, Tanokura M (2018): "Structural basis for brassinosteroid response by BIL1/BZR1." Nat Plants, 4, 771-776. doi: 10.1038/s41477-018-0255-1.

Abstract

BRZ-INSENSITIVE-LONG HYPOCOTYL 1 (BIL1)/BRASSINAZOLE-RESISTANT 1 (BZR1) is a master transcription factor of brassinosteroid (BR) signalling. The varieties of nucleobase recognition of the NN-BRRE-core motif (NNCGTG), one of variant G-box motifs, distinguish BIL1/BZR1 from basic helix-loop-helix transcription factors, underlying the specific regulation of BR-responsive genes. Here, we show the non-canonical bHLH dimer formation of BIL1/BZR1 to optimize the interaction network with DNA and the orientation of a key residue for NN-BRRE-core motif recognition.