SNAP output for PDB entry 6dsx [SNAP web server]

PDB-id

6dsx; SNAP-derived features in text and JSON formats; DNAproDB

Class

transferase-DNA

Method

X-ray (1.99 Å)

Summary

Bst DNA polymerase i post-chemistry (n+1 with datp soak) structure

Reference

Chim N, Jackson LN, Trinh AM, Chaput JC (2018): "Crystal structures of DNA polymerase I capture novel intermediates in the DNA synthesis pathway." Elife, 7. doi: 10.7554/eLife.40444.

Abstract

High resolution crystal structures of DNA polymerase intermediates are needed to study the mechanism of DNA synthesis in cells. Here we report five crystal structures of DNA polymerase I that capture new conformations for the polymerase translocation and nucleotide pre-insertion steps in the DNA synthesis pathway. We suggest that these new structures, along with previously solved structures, highlight the dynamic nature of the finger subdomain in the enzyme active site.