Summary information and primary citation

PDB-id
6dvb; SNAP-derived features in text and JSON formats; DNAproDB
Class
transferase-DNA-RNA
Method
X-ray (3.8 Å)
Summary
Crystal structure of mycobacterium tuberculosis transcription initiation complex(ecf sigma factor l) containing 5nt RNA with 5nt spacer
Reference
Lin W, Mandal S, Degen D, Cho MS, Feng Y, Das K, Ebright RH (2019): "Structural basis of ECF-sigma-factor-dependent transcription initiation." Nat Commun, 10, 710. doi: 10.1038/s41467-019-08443-3.
Abstract
Extracytoplasmic (ECF) σ factors, the largest class of alternative σ factors, are related to primary σ factors, but have simpler structures, comprising only two of six conserved functional modules in primary σ factors: region 2 (σR2) and region 4 (σR4). Here, we report crystal structures of transcription initiation complexes containing Mycobacterium tuberculosis RNA polymerase (RNAP), M. tuberculosis ECF σ factor σL, and promoter DNA. The structures show that σR2 and σR4 of the ECF σ factor occupy the same sites on RNAP as in primary σ factors, show that the connector between σR2 and σR4 of the ECF σ factor-although shorter and unrelated in sequence-follows the same path through RNAP as in primary σ factors, and show that the ECF σ factor uses the same strategy to bind and unwind promoter DNA as primary σ factors. The results define protein-protein and protein-DNA interactions involved in ECF-σ-factor-dependent transcription initiation.

Cartoon-block schematics in six views (download the tarball)

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