Summary information and primary citation
- PDB-id
- 6dvb; SNAP-derived features in text and JSON formats;
DNAproDB
- Class
- transferase-DNA-RNA
- Method
- X-ray (3.8 Å)
- Summary
- Crystal structure of mycobacterium tuberculosis transcription initiation complex(ecf sigma factor l) containing 5nt RNA with 5nt spacer
- Reference
- Lin W, Mandal S, Degen D, Cho MS, Feng Y, Das K, Ebright RH (2019): "Structural basis of ECF-sigma-factor-dependent transcription initiation." Nat Commun, 10, 710. doi: 10.1038/s41467-019-08443-3.
- Abstract
- Extracytoplasmic (ECF) σ factors, the largest class of alternative σ factors, are related to primary σ factors, but have simpler structures, comprising only two of six conserved functional modules in primary σ factors: region 2 (σR2) and region 4 (σR4). Here, we report crystal structures of transcription initiation complexes containing Mycobacterium tuberculosis RNA polymerase (RNAP), M. tuberculosis ECF σ factor σL, and promoter DNA. The structures show that σR2 and σR4 of the ECF σ factor occupy the same sites on RNAP as in primary σ factors, show that the connector between σR2 and σR4 of the ECF σ factor-although shorter and unrelated in sequence-follows the same path through RNAP as in primary σ factors, and show that the ECF σ factor uses the same strategy to bind and unwind promoter DNA as primary σ factors. The results define protein-protein and protein-DNA interactions involved in ECF-σ-factor-dependent transcription initiation.
