SNAP output for PDB entry 6en8 [SNAP web server]

PDB-id

6en8; SNAP-derived features in text and JSON formats; DNAproDB

Class

transcription

Method

X-ray (3.29 Å)

Summary

Safadr in complex with dsDNA

Reference

Wang K, Sybers D, Maklad HR, Lemmens L, Lewyllie C, Zhou X, Schult F, Brasen C, Siebers B, Valegard K, Lindas AC, Peeters E (2019): "A TetR-family transcription factor regulates fatty acid metabolism in the archaeal model organism Sulfolobus acidocaldarius." Nat Commun, 10, 1542. doi: 10.1038/s41467-019-09479-1.

Abstract

Fatty acid metabolism and its regulation are known to play important roles in bacteria and eukaryotes. By contrast, although certain archaea appear to metabolize fatty acids, the regulation of the underlying pathways in these organisms remains unclear. Here, we show that a TetR-family transcriptional regulator (FadR_Sa) is involved in regulation of fatty acid metabolism in the crenarchaeon Sulfolobus acidocaldarius. Functional and structural analyses show that FadR_Sa binds to DNA at semi-palindromic recognition sites in two distinct stoichiometric binding modes depending on the operator sequence. Genome-wide transcriptomic and chromatin immunoprecipitation analyses demonstrate that the protein binds to only four genomic sites, acting as a repressor of a 30-kb gene cluster comprising 23 open reading frames encoding lipases and β-oxidation enzymes. Fatty acyl-CoA molecules cause dissociation of FadR_Sa binding by inducing conformational changes in the protein. Our results indicate that, despite its similarity in overall structure to bacterial TetR-family FadR regulators, FadR_Sa displays a different acyl-CoA binding mode and a distinct regulatory mechanism.