SNAP output for PDB entry 6f40 [SNAP web server]

PDB-id

6f40; SNAP-derived features in text and JSON formats; DNAproDB

Class

transcription

Method

cryo-EM (3.7 Å)

Summary

RNA polymerase iii open complex

Reference

Vorlander MK, Khatter H, Wetzel R, Hagen WJH, Muller CW (2018): "Molecular mechanism of promoter opening by RNA polymerase III." Nature, 553, 295-300. doi: 10.1038/nature25440.

Abstract

RNA polymerase III (Pol III) and transcription factor IIIB (TFIIIB) assemble together on different promoter types to initiate the transcription of small, structured RNAs. Here we present structures of Pol III preinitiation complexes, comprising the 17-subunit Pol III and the heterotrimeric transcription factor TFIIIB, bound to a natural promoter in different functional states. Electron cryo-microscopy reconstructions, varying from 3.7 Å to 5.5 Å resolution, include two early intermediates in which the DNA duplex is closed, an open DNA complex, and an initially transcribing complex with RNA in the active site. Our structures reveal an extremely tight, multivalent interaction between TFIIIB and promoter DNA, and explain how TFIIIB recruits Pol III. Together, TFIIIB and Pol III subunit C37 activate the intrinsic transcription factor-like activity of the Pol III-specific heterotrimer to initiate the melting of double-stranded DNA, in a mechanism similar to that of the Pol II system.