Summary information and primary citation
- PDB-id
- 6fai; SNAP-derived features in text and JSON formats;
DNAproDB
- Class
- ribosome
- Method
- cryo-EM (3.4 Å)
- Summary
- Structure of a eukaryotic cytoplasmic pre-40s ribosomal subunit
- Reference
- Scaiola A, Pena C, Weisser M, Bohringer D, Leibundgut M, Klingauf-Nerurkar P, Gerhardy S, Panse VG, Ban N (2018): "Structure of a eukaryotic cytoplasmic pre-40S ribosomal subunit." EMBO J., 37. doi: 10.15252/embj.201798499.
- Abstract
- Final maturation of eukaryotic ribosomes occurs in the cytoplasm and requires the sequential removal of associated assembly factors and processing of the immature 20S pre-RNA Using cryo-electron microscopy (cryo-EM), we have determined the structure of a yeast cytoplasmic pre-40S particle in complex with Enp1, Ltv1, Rio2, Tsr1, and Pno1 assembly factors poised to initiate final maturation. The structure reveals that the pre-rRNA adopts a highly distorted conformation of its 3' major and 3' minor domains stabilized by the binding of the assembly factors. This observation is consistent with a mechanism that involves concerted release of the assembly factors orchestrated by the folding of the rRNA in the head of the pre-40S subunit during the final stages of maturation. Our results provide a structural framework for the coordination of the final maturation events that drive a pre-40S particle toward the mature form capable of engaging in translation.
