Summary information and primary citation
- PDB-id
- 6gh6; SNAP-derived features in text and JSON formats;
DNAproDB
- Class
- transcription
- Method
- cryo-EM (4.1 Å)
- Summary
- cryo-EM structure of bacterial RNA polymerase-sigma54 holoenzyme intermediate partially loaded complex
- Reference
- Glyde R, Ye F, Jovanovic M, Kotta-Loizou I, Buck M, Zhang X (2018): "Structures of Bacterial RNA Polymerase Complexes Reveal the Mechanism of DNA Loading and Transcription Initiation." Mol. Cell, 70, 1111-1120.e3. doi: 10.1016/j.molcel.2018.05.021.
- Abstract
- Gene transcription is carried out by multi-subunit RNA polymerases (RNAPs). Transcription initiation is a dynamic multi-step process that involves the opening of the double-stranded DNA to form a transcription bubble and delivery of the template strand deep into the RNAP for RNA synthesis. Applying cryoelectron microscopy to a unique transcription system using σ54 (σN), the major bacterial variant sigma factor, we capture a new intermediate state at 4.1 Å where promoter DNA is caught at the entrance of the RNAP cleft. Combining with new structures of the open promoter complex and an initial de novo transcribing complex at 3.4 and 3.7 Å, respectively, our studies reveal the dynamics of DNA loading and mechanism of transcription bubble stabilization that involves coordinated, large-scale conformational changes of the universally conserved features within RNAP and DNA. In addition, our studies reveal a novel mechanism of strand separation by σ54.
