SNAP output for PDB entry 6id1 [SNAP web server]

PDB-id

6id1; SNAP-derived features in text and JSON formats; DNAproDB

Class

splicing

Method

cryo-EM (2.86 Å)

Summary

cryo-EM structure of a human intron lariat spliceosome after prp43 loaded (ils2 complex) at 2.9 angstrom resolution

Reference

Zhang X, Zhan X, Yan C, Zhang W, Liu D, Lei J, Shi Y (2019): "Structures of the human spliceosomes before and after release of the ligated exon." Cell Res., 29, 274-285. doi: 10.1038/s41422-019-0143-x.

Abstract

Pre-mRNA splicing is executed by the spliceosome, which has eight major functional states each with distinct composition. Five of these eight human spliceosomal complexes, all preceding exon ligation, have been structurally characterized. In this study, we report the cryo-electron microscopy structures of the human post-catalytic spliceosome (P complex) and intron lariat spliceosome (ILS) at average resolutions of 3.0 and 2.9 Å, respectively. In the P complex, the ligated exon remains anchored to loop I of U5 small nuclear RNA, and the 3'-splice site is recognized by the junction between the 5'-splice site and the branch point sequence. The ATPase/helicase Prp22, along with the ligated exon and eight other proteins, are dissociated in the P-to-ILS transition. Intriguingly, the ILS complex exists in two distinct conformations, one with the ATPase/helicase Prp43 and one without. Comparison of these three late-stage human spliceosomes reveals mechanistic insights into exon release and spliceosome disassembly.