SNAP output for PDB entry 6ido [SNAP web server]

PDB-id

6ido; SNAP-derived features in text and JSON formats; DNAproDB

Class

transcription

Method

X-ray (3.748 Å)

Summary

Crystal structure of klebsiella pneumoniae sigma4 of sigmas fusing with the RNA polymerase beta-flap-tip-helix in complex with -35 element DNA

Reference

Lou YC, Chou CC, Yeh HH, Chien CY, Sadotra S, Hsu CH, Chen C (2020): "Structural basis for -35 element recognition by sigma4chimera proteins and their interactions with PmrA response regulator." Proteins, 88, 69-81. doi: 10.1002/prot.25768.

Abstract

In class II transcription activation, the transcription factor normally binds to the promoter near the -35 position and contacts the domain 4 of σ factors (σ₄ ) to activate transcription. However, σ₄ of σ₇₀ appears to be poorly folded on its own. Here, by fusing σ₄ with the RNA polymerase β-flap-tip-helix, we constructed two σ₄ chimera proteins, one from σ₇₀