SNAP output for PDB entry 6o1m [SNAP web server]

PDB-id

6o1m; SNAP-derived features in text and JSON formats; DNAproDB

Class

RNA binding protein-RNA-hydrolase

Method

cryo-EM (3.15 Å)

Summary

Architectural principles for hfq-crc-mediated regulation of gene expression. hfq-crc-amie 2:4:2 complex

Reference

Pei XY, Dendooven T, Sonnleitner E, Chen S, Blasi U, Luisi BF (2019): "Architectural principles for Hfq/Crc-mediated regulation of gene expression." Elife, 8. doi: 10.7554/eLife.43158.

Abstract

In diverse bacterial species, the global regulator Hfq contributes to post-transcriptional networks that control expression of numerous genes. Hfq of the opportunistic pathogen Pseudomonas aeruginosa inhibits translation of target transcripts by forming a regulatory complex with the catabolite repression protein Crc. This repressive complex acts as part of an intricate mechanism of preferred nutrient utilisation. We describe high-resolution cryo-EM structures of the assembly of Hfq and Crc bound to the translation initiation site of a target mRNA. The core of the assembly is formed through interactions of two cognate RNAs, two Hfq hexamers and a Crc pair. Additional Crc protomers are recruited to the core to generate higher-order assemblies with demonstrated regulatory activity in vivo. This study reveals how Hfq cooperates with a partner protein to regulate translation, and provides a structural basis for an RNA code that guides global regulators to interact cooperatively and regulate different RNA targets.