SNAP output for PDB entry 6ozn [SNAP web server]

PDB-id

6ozn; SNAP-derived features in text and JSON formats; DNAproDB

Class

hydrolase-RNA

Method

X-ray (1.9 Å)

Summary

Crystal structure of mus musculus (mm) endonuclease v in complex with a 23mer RNA oligo containing an inosine after a 15 min soak in 10 mm mn2+

Reference

Wu J, Samara NL, Kuraoka I, Yang W (2019): "Evolution of Inosine-Specific Endonuclease V from Bacterial DNase to Eukaryotic RNase." Mol.Cell, 76, 44. doi: 10.1016/j.molcel.2019.06.046.

Abstract

Endonuclease V (EndoV) cleaves the second phosphodiester bond 3' to a deaminated adenosine (inosine). Although highly conserved, EndoV homologs change substrate preference from DNA in bacteria to RNA in eukaryotes. We have characterized EndoV from six different species and determined crystal structures of human EndoV and three EndoV homologs from bacteria to mouse in complex with inosine-containing DNA/RNA hybrid or double-stranded RNA (dsRNA). Inosine recognition is conserved, but changes in several connecting loops in eukaryotic EndoV confer recognition of 3 ribonucleotides upstream and 7 or 8 bp of dsRNA downstream of the cleavage site, and bacterial EndoV binds only 2 or 3 nt flanking the scissile phosphate. In addition to the two canonical metal ions in the active site, a third Mn₂₊ that coordinates the nucleophilic water appears necessary for product formation. Comparison of EndoV with its homologs RNase H1 and Argonaute reveals the principles by which these enzymes recognize RNA versus DNA.