SNAP output for PDB entry 6z8k [SNAP web server]

PDB-id

6z8k; SNAP-derived features in text and JSON formats; DNAproDB

Class

replication

Method

cryo-EM (3.02 Å)

Summary

La crosse virus polymerase at elongation mimicking stage

Reference

Arragain B, Effantin G, Gerlach P, Reguera J, Schoehn G, Cusack S, Malet H (2020): "Pre-initiation and elongation structures of full-length La Crosse virus polymerase reveal functionally important conformational changes." Nat Commun, 11, 3590. doi: 10.1038/s41467-020-17349-4.

Abstract

Bunyavirales is an order of segmented negative-strand RNA viruses comprising several life-threatening pathogens against which no effective treatment is currently available. Replication and transcription of the RNA genome constitute essential processes performed by the virally encoded multi-domain RNA-dependent RNA polymerase. Here, we describe the complete high-resolution cryo-EM structure of La Crosse virus polymerase. It reveals the presence of key protruding C-terminal domains, notably the cap-binding domain, which undergoes large movements related to its role in transcription initiation, and a zinc-binding domain that displays a fold not previously observed. We capture the polymerase structure at pre-initiation and elongation states, uncovering the coordinated movement of the priming loop, mid-thumb ring linker and lid domain required for the establishment of a ten-base-pair template-product RNA duplex before strand separation into respective exit tunnels. These structural details and the observed dynamics of key functional elements will be instrumental for structure-based development of polymerase inhibitors.