SNAP output for PDB entry 6zhx [SNAP web server]

PDB-id

6zhx; SNAP-derived features in text and JSON formats; DNAproDB

Class

gene regulation

Method

cryo-EM (2.5 Å)

Summary

cryo-EM structure of the regulatory linker of alc1 bound to the nucleosome's acidic patch: nucleosome class.

Reference

Lehmann LC, Bacic L, Hewitt G, Brackmann K, Sabantsev A, Gaullier G, Pytharopoulou S, Degliesposti G, Okkenhaug H, Tan S, Costa A, Skehel JM, Boulton SJ, Deindl S (2020): "Mechanistic Insights into Regulation of the ALC1 Remodeler by the Nucleosome Acidic Patch." Cell Rep, 33, 108529. doi: 10.1016/j.celrep.2020.108529.

Abstract

Upon DNA damage, the ALC1/CHD1L nucleosome remodeling enzyme (remodeler) is activated by binding to poly(ADP-ribose). How activated ALC1 recognizes the nucleosome, as well as how this recognition is coupled to remodeling, is unknown. Here, we show that remodeling by ALC1 requires a wild-type acidic patch on the entry side of the nucleosome. The cryo-electron microscopy structure of a nucleosome-ALC1 linker complex reveals a regulatory linker segment that binds to the acidic patch. Mutations within this interface alter the dynamics of ALC1 recruitment to DNA damage and impede the ATPase and remodeling activities of ALC1. Full activation requires acidic patch-linker segment interactions that tether the remodeler to the nucleosome and couple ATP hydrolysis to nucleosome mobilization. Upon DNA damage, such a requirement may be used to modulate ALC1 activity via changes in the nucleosome acidic patches.