SNAP output for PDB entry 7lj3 [SNAP web server]

PDB-id

7lj3; SNAP-derived features in text and JSON formats; DNAproDB

Class

virus

Method

cryo-EM (2.9 Å)

Summary

Structure of human transfer RNA visualized in the cytomegalovirus, a DNA virus

Reference

Liu YT, Strugatsky D, Liu W, Zhou ZH (2021): "Structure of human cytomegalovirus virion reveals host tRNA binding to capsid-associated tegument protein pp150." Nat Commun, 12, 5513. doi: 10.1038/s41467-021-25791-1.

Abstract

Under the Baltimore nucleic acid-based virus classification scheme, the herpesvirus human cytomegalovirus (HCMV) is a Class I virus, meaning that it contains a double-stranded DNA genome-and no RNA. Here, we report sub-particle cryoEM reconstructions of HCMV virions at 2.9 Å resolution revealing structures resembling non-coding transfer RNAs (tRNAs) associated with the virion's capsid-bound tegument protein, pp150. Through deep sequencing, we show that these RNA sequences match human tRNAs, and we built atomic models using the most abundant tRNA species. Based on our models, tRNA recruitment is mediated by the electrostatic interactions between tRNA phosphate groups and the helix-loop-helix motif of HCMV pp150. The specificity of these interactions may explain the absence of such tRNA densities in murine cytomegalovirus and other human herpesviruses.