SNAP output for PDB entry 7ml3 [SNAP web server]

PDB-id

7ml3; SNAP-derived features in text and JSON formats; DNAproDB

Class

transcription

Method

cryo-EM (7.6 Å)

Summary

General transcription factor tfiih (weak binding)

Reference

Yang C, Fujiwara R, Kim HJ, Basnet P, Zhu Y, Gorbea Colon JJ, Steimle S, Garcia BA, Kaplan CD, Murakami K (2022): "Structural visualization of de novo transcription initiation by Saccharomyces cerevisiae RNA polymerase II." Mol.Cell, 82, 660-676.e9. doi: 10.1016/j.molcel.2021.12.020.

Abstract

Previous structural studies of the initiation-elongation transition of RNA polymerase II (pol II) transcription have relied on the use of synthetic oligonucleotides, often artificially discontinuous to capture pol II in the initiating state. Here, we report multiple structures of initiation complexes converted de novo from a 33-subunit yeast pre-initiation complex (PIC) through catalytic activities and subsequently stalled at different template positions. We determine that PICs in the initially transcribing complex (ITC) can synthesize a transcript of ∼26 nucleotides before transitioning to an elongation complex (EC) as determined by the loss of general transcription factors (GTFs). Unexpectedly, transition to an EC was greatly accelerated when an ITC encountered a downstream EC stalled at promoter proximal regions and resulted in a collided head-to-end dimeric EC complex. Our structural analysis reveals a dynamic state of TFIIH, the largest of GTFs, in PIC/ITC with distinct functional consequences at multiple steps on the pathway to elongation.