SNAP output for PDB entry 7ob9 [SNAP web server]

PDB-id

7ob9; SNAP-derived features in text and JSON formats; DNAproDB

Class

transcription

Method

cryo-EM (2.7 Å)

Summary

cryo-EM structure of human RNA polymerase i in elongation state

Reference

Misiaszek AD, Girbig M, Grotsch H, Baudin F, Murciano B, Lafita A, Muller CW (2021): "Cryo-EM structures of human RNA polymerase I." Nat.Struct.Mol.Biol., 28, 997-1008. doi: 10.1038/s41594-021-00693-4.

Abstract

RNA polymerase I (Pol I) specifically synthesizes ribosomal RNA. Pol I upregulation is linked to cancer, while mutations in the Pol I machinery lead to developmental disorders. Here we report the cryo-EM structure of elongating human Pol I at 2.7 Å resolution. In the exit tunnel, we observe a double-stranded RNA helix that may support Pol I processivity. Our structure confirms that human Pol I consists of 13 subunits with only one subunit forming the Pol I stalk. Additionally, the structure of human Pol I in complex with the initiation factor RRN3 at 3.1 Å resolution reveals stalk flipping upon RRN3 binding. We also observe an inactivated state of human Pol I bound to an open DNA scaffold at 3.3 Å resolution. Lastly, the high-resolution structure of human Pol I allows mapping of disease-related mutations that can aid understanding of disease etiology.