SNAP output for PDB entry 7q2x [SNAP web server]

PDB-id

7q2x; SNAP-derived features in text and JSON formats; DNAproDB

Class

cell cycle

Method

cryo-EM (3.0 Å)

Summary

cryo-EM structure of clamped s.cerevisiae condensin-DNA complex (form i)

Reference

Lee BG, Rhodes J, Lowe J (2022): "Clamping of DNA shuts the condensin neck gate." Proc.Natl.Acad.Sci.USA, 119, e2120006119. doi: 10.1073/pnas.2120006119.

Abstract

SignificanceDNA needs to be compacted to fit into nuclei and during cell division, when dense chromatids are formed for their mechanical segregation, a process that depends on the protein complex condensin. It forms and enlarges loops in DNA through loop extrusion. Our work resolves the atomic structure of a DNA-bound state of condensin in which ATP has not been hydrolyzed. The DNA is clamped within a compartment that has been reported previously in other structural maintenance of chromosomes (SMC) complexes, including Rad50, cohesin, and MukBEF. With the caveat of important differences, it means that all SMC complexes cycle through at least some similar states and undergo similar conformational changes in their head modules, while hydrolyzing ATP and translocating DNA.