Summary information and primary citation
- PDB-id
- 7uin; SNAP-derived features in text and JSON formats;
DNAproDB
- Class
- RNA binding protein-RNA-DNA
- Method
- cryo-EM (2.8 Å)
- Summary
- Cryoem structure of an group ii intron retroelement
- Reference
- Chung K, Xu L, Chai P, Peng J, Devarkar SC, Pyle AM (2022): "Structures of a mobile intron retroelement poised to attack its structured DNA target." Science, 378, 627-634. doi: 10.1126/science.abq2844.
- Abstract
- Group II introns are ribozymes that catalyze their self-excision and function as retroelements that invade DNA. As retrotransposons, group II introns form ribonucleoprotein (RNP) complexes that roam the genome, integrating by reversal of forward splicing. Here we show that retrotransposition is achieved by a tertiary complex between a structurally elaborate ribozyme, its protein mobility factor, and a structured DNA substrate. We solved cryo-electron microscopy structures of an intact group IIC intron-maturase retroelement that was poised for integration into a DNA stem-loop motif. By visualizing the RNP before and after DNA targeting, we show that it is primed for attack and fits perfectly with its DNA target. This study reveals design principles of a prototypical retroelement and reinforces the hypothesis that group II introns are ancient elements of genetic diversification.
