SNAP output for PDB entry 7utn [SNAP web server]

PDB-id

7utn; SNAP-derived features in text and JSON formats; DNAproDB

Class

RNA binding protein-RNA-DNA

Method

cryo-EM (2.74 Å)

Summary

Iscb and wrna bound to target DNA

Reference

Schuler G, Hu C, Ke A (2022): "Structural basis for RNA-guided DNA cleavage by IscB-omega RNA and mechanistic comparison with Cas9." Science, 376, 1476-1481. doi: 10.1126/science.abq7220.

Abstract

Class 2 CRISPR effectors Cas9 and Cas12 may have evolved from nucleases in IS200/IS605 transposons. IscB is about two-fifths the size of Cas9 but shares a similar domain organization. The associated ωRNA plays the combined role of CRISPR RNA (crRNA) and trans-activating CRISPR RNA