Summary information and primary citation
- PDB-id
- 7xpl; SNAP-derived features in text and JSON formats;
DNAproDB
- Class
- RNA binding protein-RNA
- Method
- X-ray (2.213 Å)
- Summary
- Crystal structure of a c-d-free RNA-guided RNA 2'-o-methyltransferase
- Reference
- Wang J, Yang Z, Ye K (2022): "Methylation guide RNAs without box C/D motifs." Rna, 28, 1597-1605. doi: 10.1261/rna.079379.122.
- Abstract
- Box C/D RNAs guide site-specific 2'-O-methylation of RNAs in archaea and eukaryotes. The defining feature of methylation guide RNAs is two sets of box C and D motifs that form kink-turn structures specifically recognized by L7Ae family proteins. Here, we engineered a new type of methylation guides that lack C/D motifs and require no L7Ae for assembly and function. We determined a crystal structure of a bipartite C/D-free guide RNA in complex with Nop5, fibrillarin and substrate in the active form at 2.2 Å resolution. The stems of new guide RNAs functionally replace C/D motifs in Nop5 binding, precisely placing the substrate for site-specific modification. We also found that the bipartite architecture and association of L7Ae with C/D motifs enhance modification when association of guide RNAs or substrates is weak. Our study provides insights into the variations, robustness and possible evolutionary path of methylation guide RNAs.
