SNAP output for PDB entry 7z0o [SNAP web server]

PDB-id

7z0o; SNAP-derived features in text and JSON formats; DNAproDB

Class

transcription

Method

cryo-EM (2.8 Å)

Summary

Structure of transcription factor uaf in complex with tbp and 35s rrna promoter DNA

Reference

Baudin F, Murciano B, Fung HKH, Fromm SA, Mattei S, Mahamid J, Muller CW (2022): "Mechanism of RNA polymerase I selection by transcription factor UAF." Sci Adv, 8, eabn5725. doi: 10.1126/sciadv.abn5725.

Abstract

Preribosomal RNA is selectively transcribed by RNA polymerase (Pol) I in eukaryotes. The yeast transcription factor upstream activating factor (UAF) represses Pol II transcription and mediates Pol I preinitiation complex (PIC) formation at the 35S ribosomal RNA gene. To visualize the molecular intermediates toward PIC formation, we determined the structure of UAF in complex with native promoter DNA and transcription factor TATA-box-binding protein (TBP). We found that UAF recognizes DNA using a hexameric histone-like scaffold with markedly different interactions compared with the nucleosome and the histone-fold-rich transcription factor IID (TFIID). In parallel, UAF positions TBP for Core Factor binding, which leads to Pol I recruitment, while sequestering it from DNA and Pol II/III-specific transcription factors. Our work thus reveals the structural basis of RNA Pol selection by a transcription factor.