SNAP output for PDB entry 8acb [SNAP web server]

PDB-id

8acb; SNAP-derived features in text and JSON formats; DNAproDB

Class

virus like particle

Method

cryo-EM (2.6 Å)

Summary

Cryoem structure of sweet potato feathery mottle virus vlp

Reference

Chase O, Javed A, Byrne MJ, Thuenemann EC, Lomonossoff GP, Ranson NA, Lopez-Moya JJ (2023): "CryoEM and stability analysis of virus-like particles of potyvirus and ipomovirus infecting a common host." Commun Biol, 6, 433. doi: 10.1038/s42003-023-04799-x.

Abstract

Sweet potato feathery mottle virus (SPFMV) and Sweet potato mild mottle virus (SPMMV) are members of the genera Potyvirus and Ipomovirus, family Potyviridae, sharing Ipomoea batatas as common host, but transmitted, respectively, by aphids and whiteflies. Virions of family members consist of flexuous rods with multiple copies of a single coat protein (CP) surrounding the RNA genome. Here we report the generation of virus-like particles (VLPs) by transient expression of the CPs of SPFMV and SPMMV in the presence of a replicating RNA in Nicotiana benthamiana. Analysis of the purified VLPs by cryo-electron microscopy, gave structures with resolutions of 2.6 and 3.0 Å, respectively, showing a similar left-handed helical arrangement of 8.8 CP subunits per turn with the C-terminus at the inner surface and a binding pocket for the encapsidated ssRNA. Despite their similar architecture, thermal stability studies reveal that SPMMV VLPs are more stable than those of SPFMV.