Summary information and primary citation

PDB-id
8csz; SNAP-derived features in text and JSON formats; DNAproDB
Class
RNA binding protein-RNA-DNA
Method
cryo-EM (3.2 Å)
Summary
Iscb and wrna bound to target DNA
Reference
Schuler G, Hu C, Ke A (2022): "Structural basis for RNA-guided DNA cleavage by IscB-omega RNA and mechanistic comparison with Cas9." Science, 376, 1476-1481. doi: 10.1126/science.abq7220.
Abstract
Class 2 CRISPR effectors Cas9 and Cas12 may have evolved from nucleases in IS200/IS605 transposons. IscB is about two-fifths the size of Cas9 but shares a similar domain organization. The associated ωRNA plays the combined role of CRISPR RNA (crRNA) and trans-activating CRISPR RNA

Cartoon-block schematics in six views (download the tarball)

PyMOL session file Download PDB file View in 3Dmol.js