SNAP output for PDB entry 8ctl [SNAP web server]

PDB-id

8ctl; SNAP-derived features in text and JSON formats; DNAproDB

Class

RNA binding protein-RNA-DNA

Method

cryo-EM (3.1 Å)

Summary

Iscb and wrna bound to target DNA (locked state)

Reference

Schuler G, Hu C, Ke A (2022): "Structural basis for RNA-guided DNA cleavage by IscB-omega RNA and mechanistic comparison with Cas9." Science, 376, 1476-1481. doi: 10.1126/science.abq7220.

Abstract

Class 2 CRISPR effectors Cas9 and Cas12 may have evolved from nucleases in IS200/IS605 transposons. IscB is about two-fifths the size of Cas9 but shares a similar domain organization. The associated ωRNA plays the combined role of CRISPR RNA (crRNA) and trans-activating CRISPR RNA