Summary information and primary citation
- PDB-id
- 8d96; SNAP-derived features in text and JSON formats;
DNAproDB
- Class
- replication
- Method
- cryo-EM (3.35 Å)
- Summary
- Human DNA polymerase alpha-primase elongation complex i bound to primer-template
- Reference
- He Q, Baranovskiy AG, Morstadt LM, Lisova AE, Babayeva ND, Lusk BL, Lim CJ, Tahirov TH (2023): "Structures of human primosome elongation complexes." Nat.Struct.Mol.Biol., 30, 579-583. doi: 10.1038/s41594-023-00971-3.
- Abstract
- The synthesis of RNA-DNA primer by primosome requires coordination between primase and DNA polymerase α subunits, which is accompanied by unknown architectural rearrangements of multiple domains. Using cryogenic electron microscopy, we solved a 3.6 Å human primosome structure caught at an early stage of RNA primer elongation with deoxynucleotides. The structure confirms a long-standing role of primase large subunit and reveals new insights into how primosome is limited to synthesizing short RNA-DNA primers.
