Summary information and primary citation
- PDB-id
-
8er8;
DSSR-derived features in text and
JSON formats; DNAproDB
- Class
- virus-DNA
- Method
- cryo-EM (2.3 Å)
- Summary
- Acheta domesticus segmented densovirus, mature virion
capsid structure
- Reference
-
Penzes JJ, Pham HT, Chipman P, Smith EW, McKenna R,
Tijssen P (2023): "Bipartite
genome and structural organization of the parvovirus
Acheta domesticus segmented densovirus." Nat
Commun, 14, 3515. doi: 10.1038/s41467-023-38875-x.
- Abstract
- Parvoviruses (family Parvoviridae) are currently
defined by a linear monopartite ssDNA genome, T = 1
icosahedral capsids, and distinct structural (VP) and
non-structural (NS) protein expression cassettes within
their genome. We report the discovery of a parvovirus with
a bipartite genome, Acheta domesticus segmented densovirus
(AdSDV), isolated from house crickets (Acheta domesticus),
in which it is pathogenic. We found that the AdSDV harbors
its NS and VP cassettes on two separate genome segments.
Its vp segment acquired a phospholipase A2-encoding gene,
vpORF3, via inter-subfamily recombination, coding for a
non-structural protein. We showed that the AdSDV evolved a
highly complex transcription profile in response to its
multipartite replication strategy compared to its
monopartite ancestors. Our structural and molecular
examinations revealed that the AdSDV packages one genome
segment per particle. The cryo-EM structures of two empty-
and one full-capsid population (3.3, 3.1 and 2.3 Å
resolution) reveal a genome packaging mechanism, which
involves an elongated C-terminal tail of the VP, "pinning"
the ssDNA genome to the capsid interior at the twofold
symmetry axis. This mechanism fundamentally differs from
the capsid-DNA interactions previously seen in
parvoviruses. This study provides new insights on the
mechanism behind ssDNA genome segmentation and on the
plasticity of parvovirus biology.
