Summary information and primary citation
- PDB-id
- 8h2h; SNAP-derived features in text and JSON formats;
DNAproDB
- Class
- RNA binding protein-RNA
- Method
- cryo-EM (3.2 Å)
- Summary
- cryo-EM structure of a group ii intron complexed with its reverse transcriptase
- Reference
- Lu Y, Liu N, Liu Y, Zheng L, Yang J, Wang J, Jia X, Zi Q, Peng H, Rao Y, Wang HW (2022): "Functionalized graphene grids with various charges for single-particle cryo-EM." Nat Commun, 13, 6718. doi: 10.1038/s41467-022-34579-w.
- Abstract
- A major hurdle for single particle cryo-EM in structural determination lies in the specimen preparation impaired by the air-water interface (AWI) and preferential particle-orientation problems. In this work, we develop functionalized graphene grids with various charges via a dediazoniation reaction for cryo-EM specimen preparation. The graphene grids are paraffin-assistant fabricated, which appear with less contaminations compared with those produced by polymer transfer method. By applying onto three different types of macromolecules, we demonstrate that the high-yield charged graphene grids bring macromolecules away from the AWI and enable adjustable particle-orientation distribution for more robust single particle cryo-EM structural determination.
