Summary information and primary citation
- PDB-id
- 8y7e; SNAP-derived features in text and JSON formats;
DNAproDB
- Class
- splicing-RNA
- Method
- cryo-EM (4.66 Å)
- Summary
- cryo-EM structure of the human minor pre-b complex (pre-precatalytic spliceosome) u12 snrnp part
- Reference
- Bai R, Yuan M, Zhang P, Luo T, Shi Y, Wan R (2024): "Structural basis of U12-type intron engagement by the fully assembled human minor spliceosome." Science, 383, 1245-1252. doi: 10.1126/science.adn7272.
- Abstract
- The minor spliceosome, which is responsible for the splicing of U12-type introns, comprises five small nuclear RNAs (snRNAs), of which only one is shared with the major spliceosome. In this work, we report the 3.3-angstrom cryo-electron microscopy structure of the fully assembled human minor spliceosome pre-B complex. The atomic model includes U11 small nuclear ribonucleoprotein (snRNP), U12 snRNP, and U4atac/U6atac.U5 tri-snRNP. U11 snRNA is recognized by five U11-specific proteins (20K, 25K, 35K, 48K, and 59K) and the heptameric Sm ring. The 3' half of the 5'-splice site forms a duplex with U11 snRNA; the 5' half is recognized by U11-35K, U11-48K, and U11 snRNA. Two proteins, CENATAC and DIM2/TXNL4B, specifically associate with the minor tri-snRNP. A structural analysis uncovered how two conformationally similar tri-snRNPs are differentiated by the minor and major prespliceosomes for assembly.
