Summary information and primary citation
- PDB-id
- 9c0j; SNAP-derived features in text and JSON formats;
DNAproDB
- Class
- transferase-DNA-RNA
- Method
- cryo-EM (3.17 Å)
- Summary
- Structure of the elongating drt2 reverse transcriptase in complex with its non-coding RNA and dntps
- Reference
- Wilkinson ME, Li D, Gao A, Macrae RK, Zhang F (2024): "Phage-triggered reverse transcription assembles a toxic repetitive gene from a noncoding RNA." Science, 386, eadq3977. doi: 10.1126/science.adq3977.
- Abstract
- Reverse transcription has frequently been co-opted for cellular functions and in prokaryotes is associated with protection against viral infection, but the underlying mechanisms of defense are generally unknown. Here, we show that in the DRT2 defense system the reverse transcriptase binds a neighboring pseudoknotted noncoding RNA. Upon bacteriophage infection, a template region of this RNA is reverse transcribed into an array of tandem repeats that reconstitute a promoter and open reading frame, allowing expression of a toxic repetitive protein and an abortive infection response. Biochemical reconstitution of this activity and cryogenic electron microscopy provide a molecular basis for repeat synthesis. Gene synthesis from a noncoding RNA is a new mode of genetic regulation in prokaryotes.
